1. Hardwood cuttings are stored at 4 ^°C for
6-8 weeks.

2.  New shoots are heat treated at the condition of 37^°C/18hrs and 22^°C/6hrs
for 4 weeks.

3.  The 0.5mm micro-shoot tips are
removed from shoots and cultured on media for 10-18 weeks.

4.  The newly grown vines are transplanted
to sterilized soils and kept in growth chamber for 3 weeks.

5.  The vines are transplanted to soils and grown in greenhouses for two years to produce enough hardwood for propagation.

1.  A foundation block of maintaining the virus-free grapevines is established in the MSFES and serves as a source of mother grapevines.

2.  The propagation of virus-free grapevines from the registered mother grapevines is enhanced in the registered stock block.

3.  The virus-free grapevines for commercial vineyards are propagated in the cooperating nurseries.

4.  The foundation block, registered block and nursery block is registered and inspected with the Missouri Department of Agriculture to comply with the Missouri Plant Law.